By Meredith N. Runner
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Extra resources for Changing Syntheses in Development
B . (1942). Lens competence in Rana pipiens, J. Exp. Zool. 9 0 , 331-347. MASUI, Y. (1966). pH-dependance of the inducing activity of lithium ion. J. Embryol. 1 5 , 371-386. a Exp: Morphol. OGI, K . (1961). Vegetalization of t h e presumptive ectoderm of Triturus gastrula by exposure t o lithium chloride solution. Embryologia 5 , 384-396. , TIMOFEEVA, Μ . , KUPRIYANOVA, N . , a n d KAFIANI, C. (1969a). Trans cription of repetitive D N A sequences in embryogenesis. Mol. Biol. (USSR) 3 , 338-347.
Zool. 1 7 4 , 101-114. TAKATA, C , a n d YAMADA, T . (1960). Endodermal tissues developed from t h e isolated newt ectoderm under the influence of guinea pig bone marrow. Embryologia 5 , 8-20. T E N CATE, G . (1953). , Amsterdam. TWITTY, V . C. (1928). Experimental studies on the ciliary action of amphibian embryos. J. Exp. Zool. 5 0 , 319-344. VILLEE, C. A. (1944). Phenogenetic studies of t h e homoeotic mutants of Drosophih melanogaster. II. T h e effects of temperature on proboscipedia. J. Exp.
A thin-layer chro matography technique was used to check the purity of the added iso 14 topes and to ascertain that C lactate was the only measurable la beled organic acid product in the serum. The glucose level in the serum was determined by the glucose oxidase method (Marks, 1959). " The amount of radioactivity added to the serum at the start represented 100%. On the assumption that cold glucose is utilized in the same manner as labeled glucose, the glucose utilization (μΐηοΐββ of glu cose per gram of protein per hour, Gu) was calculated by the follow 14 ing formula: Gu = (U X g X 55,600)IP where U is percentage of C utilized; g is mg of glucose in the medium; 55,600 is a constant for conversion to μηιοΐββ; and Ρ is total protein content of embryos and membranes in micrograms.
Changing Syntheses in Development by Meredith N. Runner